The ChIP-Seq method was used to assay chromatin fragments bound by specific or general transcription factors as described below. DNA isolated by ChIP-Seq was size-selected (~225 bp) and sequenced. Short reads of 25-36 bp were mapped to the human reference genome, and enriched regions of high read density relative to a total input chromatin control reads were identified.
The sequence reads with quality scores (fastq files) and alignment coordinates (BAM files) from these experiments are available for download.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
BAM files were downloaded from UCSC and converted to BED files. These were lifted over to hg 18 from hg19 and converted into SGA files using in-house software: