Cornelissen 2019, ERα over-expression does not accelerate development of p53-deficient mammary tumors in mice.

Genome binding profiling by high throughput sequencing of ERα in MCF7 cells.

• Raw data was downloaded from: SRP187558
• Input file format: SRA
• Download date: 21-4-2020

From H. sapiens (Feb 2009 GRCh37/hg19).

ChIP-seq data:

	Filename	Description	Feature	GEO-ID
1	GSM3640247.sga	MCF7|ESR1||rep1	ESR1	GSM3640247
2	GSM3640248.sga	MCF7|ESR1||rep2	ESR1	GSM3640248

FASTQ files were extracted from SRA files using fastq-dump (SRA toolkit v2.5.0) and mapped to the genome using Bowtie v1.2.2. SAM files were then converted to BAM using samtools v1.9 and to BED using bamToBed v2.27.0 (bedtools). BED to SGA conversion was carried out using bed2sga (ChIP-Seq v. 1.5.5).

• Cornelissen LM, Henneman L, Drenth AP, Schut E, de Bruijn R, Klarenbeek S, Zwart W, Jonkers J
  Exogenous ERα Expression in the Mammary Epithelium Decreases Over Time and Does Not Contribute to p53-Deficient Mammary Tumor Formation in Mice. J Mammary Gland Biol Neoplasia. 2019 Dec;24(4):305-321. doi: 10.1007/s10911-019-09437-z. Epub 2019 Nov 15. 31729597
• GEO series GSE127859: ERα over-expression does not accelerate development of p53-deficient mammary tumors in mice [ChIP-Seq]