Maturi 2018, Genome-wide binding of transcription factor ZEB1 in triple-negative breast cancer cells.

Description

ChIP-seq of Zinc finger E-box binding homeobox 1 (ZEB1) in the human triple-negative breast cancer cell model Hs578T.

Source

Samples

From H. sapiens (Feb 2009 GRCh37/hg19).

ChIP-seq data:

Filename Description Feature GEO-ID
1 ERS1452524.sga HS578T|input|cytokine carrier solution input
2 ERS1452525.sga HS578T|input|transforming growth factor beta 1 input
3 ERS1452526.sga HS578T|ZEB1|cytokine carrier solution ZEB1
4 ERS1452527.sga HS578T|ZEB1|transforming growth factor beta 1 ZEB1

Technical Notes

FASTQ files were extracted from ARRay Express and mapped to the genome using Bowtie2 v2.3.4.2. SAM files were then converted to BAM using samtools v1.9 and to BED using bamToBed v2.27.0 (bedtools). BED to SGA conversion was carried out using bed2sga (ChIP-Seq v. 1.5.5).

References

Last update: 1 Jul 2019