Beguelin 2013, EZH2 maps in Large B-Cell Lymphoma cell lines.

EZH2 and H3K27me3 ChIP sequencing of mouse BCL1 cell lines transduced with Y641F EZH2.

• Raw data was downloaded from: SRA website
• Input file format: SRA
• Download date: 11-9-2018

From M. musculus (July 2007 NCBI37/mm9).

ChIP-seq data:

	Filename	Description	Feature	GEO-ID
1	GSM1129365.sga	BCL1|H3K27me3|WT EZH2|	H3K27me3	GSM1129365
2	GSM1129366.sga	BCL1|H3K27me3|Y641F EZH2|	H3K27me3	GSM1129366
3	GSM1129367.sga	BCL1|H3K27me3|Y641N EZH2|	H3K27me3	GSM1129367

FASTQ files were extracted from SRA files using fastq-dump (SRA toolkit v2.5.0) and mapped to the genome using Bowtie v1.1.1. SAM files were then converted into bam using samtools v0.1.14 and to bed using bamToBed v2.12.0 (bedtools). SGA conversion was carried out using bed2sga.pl (ChIP-Seq v. 1.5.3).

• Béguelin W, Popovic R, Teater M, Jiang Y, Bunting KL, Rosen M, Shen H, Yang SN, Wang L, Ezponda T, Martinez-Garcia E, Zhang H, Zheng Y, Verma SK, McCabe MT, Ott HM, Van Aller GS, Kruger RG, Liu Y, McHugh CF, Scott DW, Chung YR, Kelleher N, Shaknovich R, Creasy CL, Gascoyne RD, Wong KK, Cerchietti L, Levine RL, Abdel-Wahab O, Licht JD, Elemento O, Melnick AM
  EZH2 is required for germinal center formation and somatic EZH2 mutations promote lymphoid transformation. Cancer Cell. 2013 May 13;23(5):677-92. doi: 10.1016/j.ccr.2013.04.011. 23680150
• GEO series GSE45982: EZH2 is required for germinal center formation and somatic EZH2 mutations promote lymphoid transformation

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