ChIP-seq analysis of NFAT1 transcription factor in CD*+ T cells. There are two version of it, the wild type (WT NFAT1) and a constitutively nuclear and therefore constitutively active (CA) and also unable to interact with AP-1 (RIT) (CA-RIT-NFAT1) present in a KO NFAT background. Some samples are also treated for 1 h with PMA and ionomycin.
Files downloaded from GEO
Input file format: SRA
From M. musculus (July 2007 NCBI37/mm9).
| Filename | Description | Feature | GEO-ID | |
| 1 | GSM1570756.sga | WT NFAT1|None | NFAT1 | GSM1570756 |
| 2 | GSM1570759.sga | WT NFAT1|PMA+Ionomycin 1h | NFAT1 | GSM1570759 |
| 3 | GSM1570757.sga | NFAT1-KO|None | NFAT1 | GSM1570757 |
| 4 | GSM1570760.sga | NFAT1-KO|PMA+Inomycin 1h | NFAT1 | GSM1570760 |
| 5 | GSM1570758.sga | NFAT1-KO CA-RIT-NFAT1|None | NFAT1 | GSM1570758 |
| 6 | GSM1570761.sga | NFAT1-KO CA-RIT-NFAT1|PMA+Ionomycin 1h | NFAT1 | GSM1570761 |
| 7 | GSM1570755.sga | Input NFAT1-KO CA-RIT-NFAT1|PMA+Ionomycin | input | GSM1570755 |
| 8 | GSM1570754.sga | Input NFAT1-KO|PMA+Inomycin 1h input | input | GSM1570754 |
| 9 | GSM1570753.sga | Input WT NFAT1|PMA+Ionomycin input | input | GSM1570753 |
| 10 | GSM1570752.sga | Input NFAT1-KO CA-RIT-NFAT1|None input | input | GSM1570752 |
| 11 | GSM1570751.sga | Input NFAT1-KO|None input | input | GSM1570751 |
| 12 | GSM1570750.sga | Input WT NFAT1|None input | input | GSM1570750 |
Raw sequences were mapped to mouse genome using bowtie and converted into SGA file format using samtools (download from http://samtools.sourceforge.net/ ) and ChIP-Seq tools (download from http://sourceforge.net/projects/chip-seq/ )