Young 2011, ChIP-seq analysis of H3K27me3 and PolII in 2 cell lines.

ChIP-seq against H3K27me3 and, RNApol-II in mouse embryonic stem (ES) cells and haemopoietic cell line (G1ME). As a control, whole cell extract (WCE/input) was also sequenced for both cell types without the inclusion of an antibody.

• Raw data was downloaded from GEO series: GSE27967
• Input file format: BAM

From M. musculus (July 2007 NCBI37/mm9).

ChIP-seq data:

	Filename	Description	Feature	GEO-ID
1	GSM691591_ES_H3K27me3.sga	ES H3K27me3	H3K27me3	GSM691591
2	GSM691592_ES_PolII.sga	ES RNApol-II	PolII	GSM691592
3	GSM691593_ES_none.sga	ES WCE	none	GSM691593
4	GSM691594_H3K27me3.sga	G1ME H3K27me3	H3K27me3	GSM691594
5	GSM691595_PolII.sga	G1ME RNApol-II	PolII	GSM691595
6	GSM691596_G1ME_none.sga	G1ME WCE	none	GSM691596

BAM files were converted into BED using bamToBed v2.12.0 (bedtools). SGA conversion was carried out using bed2sga.pl (ChIP-Seq v. 1.5.2).

• Young MD, Willson TA, Wakefield MJ, Trounson E, Hilton DJ, Blewitt ME, Oshlack A, Majewski IJ
  ChIP-seq analysis reveals distinct H3K27me3 profiles that correlate with transcriptional activity. Nucleic Acids Res. 2011 Sep 1;39(17):7415-27. doi: 10.1093/nar/gkr416. Epub 2011 Jun 7. 21652639
• GEO series GSE27967: ChIP-seq analysis reveals distinct H3K27me3 profiles associated with gene regulation [ChIP-seq]