Genome-wide mapping of various histone modifications.

Description

Genome-wide mapping of H3K27me3, H3K36me3, H3K4me3 and H3K9ac in shoot and root tissues for the B73 cultivar.

Source

Samples

From Z. mays (Oct 2013 B73 RefGen_v3/zm3).

ChIP-seq data:

Filename Description Feature GEO-ID
1 GSM381721.sga root|H3K27me3|rep H3K27me3 GSM381721
2 GSM381723.sga root|H3K36me3|rep H3K36me3 GSM381723
3 GSM381717.sga root|H3K4me3|rep H3K4me3 GSM381717
4 GSM381719.sga root|H3K9ac|rep H3K9ac GSM381719
5 GSM381699.sga shoot|H3K27me3|rep H3K27me3 GSM381699
6 GSM381701.sga shoot|H3K36me3|rep H3K36me3 GSM381701
7 GSM381695.sga shoot|H3K4me3|rep H3K4me3 GSM381695
8 GSM381697.sga shoot|H3K9ac|rep H3K9ac GSM381697

Technical Notes

FASTQ files were extracted from SRA files using fastq-dump (SRA toolkit v2.5.0) and mapped to the genome using Bowtie v0.12.8. SAM files were then converted into bam using samtools v0.1.14 and to bed using bamToBed v2.12.0 (bedtools). SGA conversion was carried out using bed2sga.pl (ChIP-Seq v. 1.5.2).

References

Last update: 1 Oct 2018